Line immunoassay for the confirmation and discrimination of antibodies to HIV-1, HIV-1 group O and HIV-2 in human serum and plasma.
Antibodies to human immunodeficiency virus (HIV) have been detected in most patients with acquired immunodeficiency syndrome (AIDS) and AIDS-related complex (ARC), and in many asymptomatic individuals belonging to high-risk groups. Enzyme-linked immunosorbent assays (ELISA) are widely used to screen human blood and plasma samples for the presence of these HIV antibodies.
However, since false-positive reactions have frequently been observed with current ELISA tests, it is strongly recommended to confirm repeatedly reactive samples by use of other reliable techniques (such as the INNO-LIA HIV I/II Score). Recombinant proteins and synthetic peptides from HIV-1 and HIV-2, and a synthetic peptide from HIV-1 group O, are coated as discrete lines on a nylon strip with plastic backing.
The INNO-LIA HIV I/II Score assay contains sequences derived from HIV-O sequences to allow the optimal detection of infections caused by this group of viruses. To allow the broad spectrum detection of all HIV infections, the INNO-LIA HIV I/II Score assay also contains separate lines for the differentiation of HIV-1 and HIV-2 infections.
This is important, as HIV-2 is less virulent, resulting in a better prognosis for the patient.
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