Line probe assay, based on the reverse hybridisation principle, designed for the identification of 32 different genotypes of the human papillomavirus (HPV).

• Individual identification of 32 genotypes.
• One genotype = one probe line.
• Ready-to-use mastermix.
• Automation.
• Primer mix for RT-PCR application.
• Cervical cells and first-void urine (CE).
• FFPE samples (RUO).

Features and benefits

Full genotyping

• Simultaneous detection of 32 HPV genotypes.
• SPF10 Plus provides high test sensitivity due to the precision of the short 65-base pair PCR product.
• SPF10 Plus permits simultaneous detection of multiple genotypes in a single sample.

Built-in controls

• Special HPV control lines to confirm and detect the presence of a broad range of mucosal HPV genotypes plus additional controls to monitor sample processing (hDNA line, conjugate control line).

Streamlining

• Easy and ready-to-use master mix, with Taq DNA polymerase provided for minimum manipulation steps and hands-on time.

CE Applications:

• Cervical cells collected in Surepath medium or PreservCyt medium.
• First-void urine with preservative.

RUO Applications:

• Paraffin-embedded (FFPE) material.
• HPV Primer Mix SPF10 Plus for Real-Time PCR application.

Automation

• Auto-LiPA™ 48: up to 48 tests per run from sample incubation to colour development.
• AutoBlot 3000H: up to 20 tests per run from sample incubation to colour development.
• TENDIGO®: up to 10 tests per run from sample incubation to colour development.
• Results within +/- 3 hours.
• Minimum hands-on time, accurate results.
• Interpretation software for complete traceability.

For more information, visit Fujirebio.com.